Clayton A. Wiley, MD PhD

Professor, Pathology

Contact

M8715 South Tower, UPMC Presbyterian Hospital
412-647-0765
F: 412-647-5602
wiley1@pitt.edu
Website >

Education

MD, University of California, San Diego (1981)
PhD, University of California, San Diego (1981)

Focus

Examining mechanisms of neurodegeneration associated with Alzheimer's Disease, AIDS, West Nile Virus and influenza infection.

Research Summary

Dr. Wiley's research concentrates on the pathogenesis of neurodegenerative diseases and in particular on viral and age induced nervous system diseases. Viruses damage the nervous system either by direct infection of neural cells or by secondary effects of an immune response. In recent years his studies have focused on central nervous system retroviral infections and have pioneered the use of molecular and imaging techniques to quantitatively assess viral burden. This same technology is now being applied to quantify neurological damage and the immune / neuroinflammatory response with specific attention focused on disruption of the extracellular matrix and how this leads to synaptic damage. Using MRI and PET he is investigating the evolution of blood brain barrier defects and macrophage trafficking in mediating neurological disease. Trainees in Dr. Wiley's laboratory have the opportunity to learn a wide variety of techniques related to studying degeneration of the nervous system. Whole organism techniques include; PET, MRI, brain harvesting and sampling, and small animal perfusion, histological techniques include; immunocytochemistry, in situ hybridization and ultrastructural analysis. Training on laser confocal and electron microscopes is available. Protein techniques include; antibody purification and tagging. Molecular techniques include; RNA and DNA extraction from tissues, competitive PCR probe construction and quantitation. Lab rotation projects involve quantitation of mRNA and protein expression in human nervous system tissue in vivo or in vitro.

Publications

Bissel SJ, Kofler J, Nyaundi J, Mickey-Corb M, Wisniewski SR, Wiley CA. Cerebral Spinal Fluid Biomarkers of Primate Lentiviral Encephalitis. J Neuroimmune Pharmacol (2016) 11(2):332-347. PMCID: in process
 
Wiley, C. A, Bissel, S. J., Lesniak, A., Dixon, C. E., Franks, J., Stolz, D. B., Sun, M., Wang, G., Switzer III, R. C., Kochanek, P. M. and Murdoch, G. H. Ultrastructure of diaschisis lesions following traumatic brain injury. J Neurotrauma 2016 In Press. PMID: 26914973.
 
CH Chiang, CT Chu & CA Wiley. (2015) A 67 year old man with multiple sclerosis and new onset cerebellar lesions. (progressive multifocal leucoencephalopathy with superimposed immune reconstitution inflammatory syndrome). Electronically published March 15, 2015. Brain Pathol, 25:507-508, PMID: 26095593.
 
Bissel SJ, Auer R, Chiang C-H, Kofler J, Murdoch GH, Nix WA, Painter M, Richer M, Startelet H and Wiley CA: Human Parechovirus 3 Meningitis and Fatal Leukoencephalopathy. J Neuropathology and Experimental Neurology (2015) PMID: 26115191.
 
Mardekian SK, Fortuna D, Nix A, Bhatti T, Wiley CA, Flanders A, Urtecho, J, Sloane, J, Ahmad, J and Curtis, M. T. Severe Human Parechovirus Type 3 Myocarditis and Encephalitis in an Adolescent with Hypogammaglobulinemia. International Journal of Infectious Diseases 2015. doi: 10.1016/j.ijid.2015.05.008. PMID: 25975649.
 
Wiley CA, Bonneh-Barkay D, Dixon CE, Lesniak A, Wang G, Bissel SJ and Kochanek PM: Role for mammalian chitinase 3-like protein 1 in traumatic brain injury. Neuropathology (2015) 35:95-106. PMID: 25377763

 

Bonneh-Barkay, D., Wang, G., Starkey, A., Hamilton, R.L. and Wiley, C. In vivo CHI3L1 (YKL-40) expression in astrocytes in acute and chronic neurological diseases. Journal of Neuroinflammation 7:34 2010 In Press.

Bonneh-Barkay, D., Zagadailov, P., Zou, H., Niyonkuru, C., Figley, M., Starkey, A., Wang, G., Bissel, S.J., Wiley, C.A. and Wagner, A. YKL-40 expression in traumatic brain injury - an initial analysis. J Neurotrauma 27:1-9 (2010b).

Venneti, S., Lopresti, B.J., Wang, G., Hamilton, R.L., Mathis, C.A., Klunk, W.E., Apte, U.M. and Wiley, C.A. PK11195 labels activated microglia in Alzheimer's disease and in vivo in a mouse model using PET. Neurobiology of Aging. 30: 1217-1226, 2009.

Wiley, C.A., Lopresti, B.J., Venneti, S., Price, J., Klunk, W.E., DeKosky, S. and Mathis, C.A. [11C]PIB and [11C](R)-PK11195 PET imaging in Alzheimer's disease. Arch Neurol 66: 60-67, 2009. (PMCID: PMC2666881)

Bonneh-Barkay, D. and Wiley, C.A. Brain extracellular matrix in neurodegeneration. Brain Pathology 19:573-585, 2009 NIHMSID # 100656

Venneti, S., Wang, G. and Wiley, C.A. The high affinity peripheral benzodiazepine receptor ligand DAA1106 binds to activated and infected brain macrophages in areas of synaptic degeneration: Implications for PET imaging of neuroinflammation in lentiviral encephalitis. Neurobiology of Disease 29: 232-241, 2008. (PMCID: PMC2258458)

Bissel, S.J., Wang, G., Bonneh-Barkay, D., Starkey, A., Trichel, A.M., Murphey-Corb, M. and Wiley, C.A. Systemic and brain macrophage infections in relation to the development of simian immunodeficiency virus encephalitis. Journal of Virology 82: 5031-5042, 2008. (PMCID: PMC2346725)