Clayton A. Wiley, MD, PhD

  • Professor, Pathology

Phone

412-647-0765

E-mail

wiley1@pitt.edu

Education & Training

MD, University of California, San Diego (1981)
PhD, University of California, San Diego (1981)

Location

M8715 South Tower, UPMC Presbyterian Hospital

Research Interest Summary

Examining mechanisms of neurodegeneration associated with Alzheimer's Disease, AIDS, West Nile Virus and influenza infection.

Dr. Wiley's research concentrates on the pathogenesis of neurodegenerative diseases and in particular on viral and age induced nervous system diseases. Viruses damage the nervous system either by direct infection of neural cells or by secondary effects of an immune response. In recent years his studies have focused on central nervous system retroviral infections and have pioneered the use of molecular and imaging techniques to quantitatively assess viral burden. This same technology is now being applied to quantify neurological damage and the immune / neuroinflammatory response with specific attention focused on disruption of the extracellular matrix and how this leads to synaptic damage. Using MRI and PET he is investigating the evolution of blood brain barrier defects and macrophage trafficking in mediating neurological disease. Trainees in Dr. Wiley's laboratory have the opportunity to learn a wide variety of techniques related to studying degeneration of the nervous system. Whole organism techniques include; PET, MRI, brain harvesting and sampling, and small animal perfusion, histological techniques include; immunocytochemistry, in situ hybridization and ultrastructural analysis. Training on laser confocal and electron microscopes is available. Protein techniques include; antibody purification and tagging. Molecular techniques include; RNA and DNA extraction from tissues, competitive PCR probe construction and quantitation. Lab rotation projects involve quantitation of mRNA and protein expression in human nervous system tissue in vivo or in vitro.

Bissel SJ, Carter CE, Wang G, Johnson SK, Lashua LP, Kelvin A, Wiley CA, Ghedin E, Ross TM. Age-related pathology associated with H1N1 A/California/07/2009 influenza virus infection. The American Journal of Pathology. PMID 31585069 DOI: 10.1016/j.ajpath.2019.08.017 

 

Emery SP, Green S, Murdoch G and Wiley CA; Histologic appearance of iatrogenic obstructive hydrocephalus in the fetal lamb model. Fetal Diagnosis and Therapy 2019 InPress

 

Bissel, SJ, Gurnsey, K, Jadema, HP, Smith, NF, Wang, G, Bradberry, CW and Wiley, CA : Aged Chineseoriginrhesus macaques infected with SIV develop marked viremia in absence of clinical disease,inflammation or cognitive impairment. Retrovirology (2018) 15 (1):17 PMID 29391069

 

Chimelli L, Pone SM, Avvad-Portari E, Vasconcelos ZFM, Zin AA, Cunha DP, Thompson NR, MoreiraREL, Wiley CA and Pone, MVS: Persistence of Zika Virus After Birth: Clinical, Virological,Neuroimaging, and Neuropathological Documentation in a 5-Month Infant With Congenital ZikaSyndrome. J Neuropathol Exp Neurol Vol. 77, No. 3, March 2018, pp. 193–198 PMID 29346650234.

 

Thomas DL, Manners J, Marker D, Mettenburg J, Murdoch G, Stevens B, Wang G, Wiley C CD8-PositiveT-Cell Leukoencephalitis With Astrocytopathy Clinically Presenting as Neuromyelitis Optica. Journal ofneuropathology and experimental neurology. (2017) 76(5):347-57. PMID 28340257

 

Bissel SJ, Kofler J, Nyaundi J, Mickey-Corb M, Wisniewski SR, Wiley CA. Cerebral Spinal Fluid Biomarkers of Primate Lentiviral Encephalitis. J Neuroimmune Pharmacol (2016) 11(2):332-347. PMCID: in process

 

Wiley CA , Bhardwaj N, Ross TM, Bissel SJ. Emerging Infections of CNS: Avian Influenza A Virus, RiftBissel SJ, Kofler J, Nyaundi J, Mickey-Corb M, Wisniewski SR, Wiley CA . Cerebral Spinal FluidBiomarkers of Primate Lentiviral Encephalitis. J Neuroimmune Pharmacol (2016) 11(2):332-347. PMID:27059917 PMCID: 4871628

 

Wiley, C. A, Bissel, S. J., Lesniak, A., Dixon, C. E., Franks, J., Stolz, D. B., Sun, M., Wang, G., Switzer III, R. C., Kochanek, P. M. and Murdoch, G. H. Ultrastructure of diaschisis lesions following traumatic brain injury. J Neurotrauma 2016 In Press. PMID: 26914973.

 

CH Chiang, CT Chu & CA Wiley. (2015) A 67 year old man with multiple sclerosis and new onset cerebellar lesions. (progressive multifocal leucoencephalopathy with superimposed immune reconstitution inflammatory syndrome). Electronically published March 15, 2015. Brain Pathol, 25:507-508, PMID: 26095593.

 

Bissel SJ, Auer R, Chiang C-H, Kofler J, Murdoch GH, Nix WA, Painter M, Richer M, Startelet H and Wiley CA: Human Parechovirus 3 Meningitis and Fatal Leukoencephalopathy. J Neuropathology and Experimental Neurology (2015) PMID: 26115191.

 

Mardekian SK, Fortuna D, Nix A, Bhatti T, Wiley CA, Flanders A, Urtecho, J, Sloane, J, Ahmad, J and Curtis, M. T. Severe Human Parechovirus Type 3 Myocarditis and Encephalitis in an Adolescent with Hypogammaglobulinemia. International Journal of Infectious Diseases 2015. doi: 10.1016/j.ijid.2015.05.008. PMID: 25975649.

 

Wiley CA, Bonneh-Barkay D, Dixon CE, Lesniak A, Wang G, Bissel SJ and Kochanek PM: Role for mammalian chitinase 3-like protein 1 in traumatic brain injury. Neuropathology (2015) 35:95-106. PMID: 25377763

 

Bissel SJ, Wang G, Carter DM, Crevar CJ, Ross TM, Wiley CA . H1N1, but Not N3N2, Influenza A VirusInfection Protects Ferrets from H5N1 Encephalitis. J Virol (2014) 88(6):3077-3091. PMCID:PMC3957958; PMID 24371072

 

Bissel SJ, Winkler CC, DelTondo J, Wang G, Williams K and Wiley CA . Coxsackievirus B4 myocarditisand meningoencephalitis in newborn twins. Neuropathology (2014) 34, 429–437 Apr 7. doi:10.1111/neup.12121. PMCID: PMC4188796; PMID 2470228

 

Bissel, SJ, Gurnsey, K, Jadema, HP, Smith, NF, Wang, G, Bradberry, CW and Wiley, CA : Aged Chineseoriginrhesus macaques infected with SIV develop marked viremia in absence of clinical disease,inflammation or cognitive impairment. Retrovirology (2018) 15 (1):17 PMID 29391069

 

Chimelli L, Pone SM, Avvad-Portari E, Vasconcelos ZFM, Zin AA, Cunha DP, Thompson NR, MoreiraREL, Wiley CA and Pone, MVS: Persistence of Zika Virus After Birth: Clinical, Virological,Neuroimaging, and Neuropathological Documentation in a 5-Month Infant With Congenital ZikaSyndrome. J Neuropathol Exp Neurol Vol. 77, No. 3, March 2018, pp. 193–198 PMID 29346650234.

 

Thomas DL, Manners J, Marker D, Mettenburg J, Murdoch G, Stevens B, Wang G, Wiley C CD8-PositiveT-Cell Leukoencephalitis With Astrocytopathy Clinically Presenting as Neuromyelitis Optica. Journal ofneuropathology and experimental neurology. (2017) 76(5):347-57. PMID 28340257

 

Bissel SJ, Kofler J, Nyaundi J, Mickey-Corb M, Wisniewski SR, Wiley CA. Cerebral Spinal Fluid Biomarkers of Primate Lentiviral Encephalitis. J Neuroimmune Pharmacol (2016) 11(2):332-347. PMCID: in process

 

Wiley CA , Bhardwaj N, Ross TM, Bissel SJ. Emerging Infections of CNS: Avian Influenza A Virus, RiftBissel SJ, Kofler J, Nyaundi J, Mickey-Corb M, Wisniewski SR, Wiley CA . Cerebral Spinal FluidBiomarkers of Primate Lentiviral Encephalitis. J Neuroimmune Pharmacol (2016) 11(2):332-347. PMID:27059917 PMCID: 4871628

 

Wiley, C. A, Bissel, S. J., Lesniak, A., Dixon, C. E., Franks, J., Stolz, D. B., Sun, M., Wang, G., Switzer III, R. C., Kochanek, P. M. and Murdoch, G. H. Ultrastructure of diaschisis lesions following traumatic brain injury. J Neurotrauma 2016 In Press. PMID: 26914973.

 

CH Chiang, CT Chu & CA Wiley. (2015) A 67 year old man with multiple sclerosis and new onset cerebellar lesions. (progressive multifocal leucoencephalopathy with superimposed immune reconstitution inflammatory syndrome). Electronically published March 15, 2015. Brain Pathol, 25:507-508, PMID: 26095593.

 

Bissel SJ, Auer R, Chiang C-H, Kofler J, Murdoch GH, Nix WA, Painter M, Richer M, Startelet H and Wiley CA: Human Parechovirus 3 Meningitis and Fatal Leukoencephalopathy. J Neuropathology and Experimental Neurology (2015) PMID: 26115191.

 

Mardekian SK, Fortuna D, Nix A, Bhatti T, Wiley CA, Flanders A, Urtecho, J, Sloane, J, Ahmad, J and Curtis, M. T. Severe Human Parechovirus Type 3 Myocarditis and Encephalitis in an Adolescent with Hypogammaglobulinemia. International Journal of Infectious Diseases 2015. doi: 10.1016/j.ijid.2015.05.008. PMID: 25975649.

 

Wiley CA, Bonneh-Barkay D, Dixon CE, Lesniak A, Wang G, Bissel SJ and Kochanek PM: Role for mammalian chitinase 3-like protein 1 in traumatic brain injury. Neuropathology (2015) 35:95-106. PMID: 25377763

 

Bissel SJ, Wang G, Carter DM, Crevar CJ, Ross TM, Wiley CA . H1N1, but Not N3N2, Influenza A VirusInfection Protects Ferrets from H5N1 Encephalitis. J Virol (2014) 88(6):3077-3091. PMCID:PMC3957958; PMID 24371072

 

Bissel SJ, Winkler CC, DelTondo J, Wang G, Williams K and Wiley CA . Coxsackievirus B4 myocarditisand meningoencephalitis in newborn twins. Neuropathology (2014) 34, 429–437 Apr 7. doi:10.1111/neup.12121. PMCID: PMC4188796; PMID 2470228